Insertion junction: LMJ.RY0402.232799_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.232799
Chromosome:chromosome 10
Location:1542666
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g429050 XPG2 DNA repair 5'-3' exonuclease; (1 of 1) K10846 - DNA excision repair protein ERCC-5 (ERCC5, XPG, RAD2) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCGGCAGTTCTGCAGGTGTGTATGTATG
Internal bar code:TGAAAAGCTTCTCGACGCGAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:3.64583
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:185
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ACACACACACACACACCACG
Suggested primer 2:TTGTGGGTGTCTTGGTGTGT