| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.232799 |
| Chromosome: | chromosome 10 |
| Location: | 1542807 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g429050 | XPG2 | DNA repair 5'-3' exonuclease; (1 of 1) K10846 - DNA excision repair protein ERCC-5 (ERCC5, XPG, RAD2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGCGCTGCTGCGCCGGCGTGTCGTCAGG |
| Internal bar code: | GGAGGCAAAGCAAGGTTAGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 396 |
| LEAP-Seq percent confirming: | 88.4259 |
| LEAP-Seq n confirming: | 191 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACACACACACACACCACG |
| Suggested primer 2: | TTGTGGGTGTCTTGGTGTGT |