Insertion junction: LMJ.RY0402.232804_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.232804
Chromosome:chromosome 14
Location:1498705
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g618400 ABCA3 (1 of 4) 3.6.3.29 - Molybdate-transporting ATPase; ABC transporter 3 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGGTGCTGTGGTGACCCCACGCAGCCCGC
Internal bar code:GGGGATTGATAATAACAGTGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:621
LEAP-Seq percent confirming:98.753
LEAP-Seq n confirming:2059
LEAP-Seq n nonconfirming:26
LEAP-Seq n unique pos:44

Suggested primers for confirmation by PCR

Suggested primer 1:CGCATGACAGGATAAGCAGA
Suggested primer 2:CTGGAAGGTAGAGAGGTGCG