| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.232806 |
| Chromosome: | chromosome 3 |
| Location: | 6619521 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g196600 | RAD17 | (1 of 1) PTHR12172//PTHR12172:SF0 - CELL CYCLE CHECKPOINT PROTEIN RAD17 // RAD17; presequence translocase-associated protein import motor subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAAGCCGGCTGCTCAGCTCAGCTCCGCC |
| Internal bar code: | GGAGGTTCGCTAGCCGTCTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 611 |
| LEAP-Seq percent confirming: | 98.8868 |
| LEAP-Seq n confirming: | 4264 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 71 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGATGCTGATGTCGCAGATG |
| Suggested primer 2: | CTGACGTCAAGGTGGTGCTA |