Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.232815 |
Chromosome: | chromosome_10 |
Location: | 941502 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre10.g424600 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CATGCCGAGACCCAGTACTAGAGCGCGGAG |
Internal bar code: | ACTCGCAATCAAACGTCCAGCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 310 |
LEAP-Seq percent confirming: | 98.6637 |
LEAP-Seq n confirming: | 443 |
LEAP-Seq n nonconfirming: | 6 |
LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTTCCTCTCACGCAAACCTC |
Suggested primer 2: | ATCTCGGGTGCTAATGGTTG |