Insertion junction: LMJ.RY0402.232824_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.232824
Chromosome:chromosome 16
Location:277804
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g694200 MMP15 Matrix-metalloproteinase-like protein; (1 of 40) 3.4.24.38 - Gametolysin / Lysin 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACATGTCTAGCAAACTGCTCGATGCGGTT
Internal bar code:GTGGCGTGTCGACGGCGTTGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:432
LEAP-Seq percent confirming:18.8811
LEAP-Seq n confirming:243
LEAP-Seq n nonconfirming:1044
LEAP-Seq n unique pos:35

Suggested primers for confirmation by PCR

Suggested primer 1:TGCCGGTAGGGCTTATATTG
Suggested primer 2:CGCACAGTAAAGTTGGGGAT