Insertion junction: LMJ.RY0402.232853_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.232853
Chromosome:chromosome 10
Location:1454300
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g428650 CDPKK1,CDPKKK1 (1 of 3) K07359 - calcium/calmodulin-dependent protein kinase kinase (CAMKK); Calcium/calmodulin-dependent protein kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCCACCAGCACCATACCATCCCATCCCCG
Internal bar code:GTATTTGAAGGGGACAATTGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:218
LEAP-Seq percent confirming:98.5958
LEAP-Seq n confirming:4283
LEAP-Seq n nonconfirming:61
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCTGCTGGTGATGGAGTA
Suggested primer 2:GCTGCATTATTGCGTAGGGT