| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.232860 |
| Chromosome: | chromosome 3 |
| Location: | 1928486 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g155350 | PPO4 | Pyridoxamine 5'-phosphate oxidase-like protein; (1 of 1) 1.14.13.142 - 3-ketosteroid 9-alpha-monooxygenase / 3-ketosteroid 9-alpha-hydroxylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCTGAATGTACTTGGGGCAGTTGCCAAA |
| Internal bar code: | TTGGAGCTGGTCCTGTTCCTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 288 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 869 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAATCCTGTGCTTCCCAAC |
| Suggested primer 2: | TAGGAAGGTGTGGGGAAGTG |