| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.232870 |
| Chromosome: | chromosome 13 |
| Location: | 4977853 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g606350 | (1 of 1) PF12816 - Golgi CORVET complex core vacuolar protein 8 (Vps8) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGGGCGGCAGCCCCCGCAGCCCCCGCAC |
| Internal bar code: | TGGGTTCCTTTCTGACAACGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 159 |
| LEAP-Seq percent confirming: | 99.737 |
| LEAP-Seq n confirming: | 2655 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGAGGGCTGAGACGAAGGT |
| Suggested primer 2: | GTTTGCCCAATGGGAGTCTA |