Insertion junction: LMJ.RY0402.232872_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.232872
Chromosome:chromosome 2
Location:4945597
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g103800 (1 of 31) IPR003590 - Leucine-rich repeat, ribonuclease inhibitor subtype CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCCAGTCCTGCGGCGCGGGCCCGGTGCC
Internal bar code:CGTCCCTCAACATACCTGGGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:583
LEAP-Seq percent confirming:99.2132
LEAP-Seq n confirming:3657
LEAP-Seq n nonconfirming:29
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:CGTCCAAAGACTCGTAAGGC
Suggested primer 2:CCTCTTGTAACCCGTGCAAT