Insertion junction: LMJ.RY0402.232882_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.232882
Chromosome:chromosome 7
Location:1888462
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g325739 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCTAGACGACGGCGGTGACGGACAGGCG
Internal bar code:CTCCTTGGGGGATGAATCGACCA

Confirmation - LEAP-Seq

LEAP-Seq distance:492
LEAP-Seq percent confirming:91.9571
LEAP-Seq n confirming:2058
LEAP-Seq n nonconfirming:180
LEAP-Seq n unique pos:95

Suggested primers for confirmation by PCR

Suggested primer 1:AAGGGGCTGACAATCAACAC
Suggested primer 2:CTGTTGGGGCGAGATGTAAT