| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.232893 |
| Chromosome: | chromosome 11 |
| Location: | 274552 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467559 | (1 of 1) IPR003590//IPR006553 - Leucine-rich repeat, ribonuclease inhibitor subtype // Leucine-rich repeat, cysteine-containing subtype | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGCGGGTCCACTTCCACTTCCACTTCCA |
| Internal bar code: | CTAATTTGACTTTCACTACTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 17 |
| LEAP-Seq percent confirming: | 92.3077 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATATGAAGTGAAGTGCCGGG |
| Suggested primer 2: | GTGACGTTGGTGTGGTTGAG |