| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.232912 |
| Chromosome: | chromosome 12 |
| Location: | 7157494 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g558950 | (1 of 1) IPR000104//IPR009071 - Antifreeze protein, type I // High mobility group box domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGACGACACCCACCGTTGCCGTGGCATCT |
| Internal bar code: | CCGCAGTACAGTCTGACCGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 825 |
| LEAP-Seq percent confirming: | 99.7984 |
| LEAP-Seq n confirming: | 495 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCGTTCTGACCGCTTTTT |
| Suggested primer 2: | GTCATCGTCTTCGTCGTCAG |