| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.232924 |
| Chromosome: | chromosome 16 |
| Location: | 5900827 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g677150 | (1 of 2) 2.4.1.122 - Glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCAAGTGGATGCTGTACGGAGACGATGG |
| Internal bar code: | AGACCGCCATTCAAGGCGGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 557 |
| LEAP-Seq percent confirming: | 96.9461 |
| LEAP-Seq n confirming: | 1492 |
| LEAP-Seq n nonconfirming: | 47 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGACGTAAACGACACCTC |
| Suggested primer 2: | ATGCTAGGGTGGTAGCCCTT |