Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.232935 |
Chromosome: | chromosome_1 |
Location: | 1751521 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre01.g009400 | NSS2 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CCCATGCCCACGTAACGCCGCCCCGTTCAT |
Internal bar code: | GCACGGGTGGTAGAAGGGGGAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 332 |
LEAP-Seq percent confirming: | 99.4875 |
LEAP-Seq n confirming: | 12618 |
LEAP-Seq n nonconfirming: | 65 |
LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCCTGTGAGACGCACAAGTA |
Suggested primer 2: | CACACACATACTGATCCGCC |