Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.232943 |
Chromosome: | chromosome 12 |
Location: | 7297420 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g557600 | ADK4 | Adenylate kinase | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGTCCAACGCTATCCTAGGTCTCATATC |
Internal bar code: | CTTTCCCTCCCAGTGGATCAGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 371 |
LEAP-Seq percent confirming: | 97.7686 |
LEAP-Seq n confirming: | 19016 |
LEAP-Seq n nonconfirming: | 434 |
LEAP-Seq n unique pos: | 76 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CACGTACTCCATCCATGACG |
Suggested primer 2: | CACACACACACACACCAGGA |