| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.232948 |
| Chromosome: | chromosome 2 |
| Location: | 3918586 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g096350 | DAO1 | D-amino-acid oxidase, FAD-containing; (1 of 1) K00273 - D-amino-acid oxidase (DAO, aao) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCTACTACATCATCCCAAACAGGCGAGT |
| Internal bar code: | CGGCATACGGACATCGGCTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1071 |
| LEAP-Seq percent confirming: | 99.5854 |
| LEAP-Seq n confirming: | 11771 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTGTCGTCCGCTTAGCTT |
| Suggested primer 2: | CCAGCCCAAACTTAACCAAA |