| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.232953 |
| Chromosome: | chromosome 3 |
| Location: | 5539051 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g186200 | CDS1,PCT1 | Phosphatidate cytidylyltransferase; (1 of 1) PTHR13773:SF8 - PHOSPHATIDATE CYTIDYLYLTRANSFERASE, PHOTORECEPTOR-SPECIFIC | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTACATGGCCGCTAGGTCCCAAGGACTTG |
| Internal bar code: | CCAGCTTTATGGCCGGTTTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 456 |
| LEAP-Seq percent confirming: | 92.9974 |
| LEAP-Seq n confirming: | 3267 |
| LEAP-Seq n nonconfirming: | 246 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCAGTAGCCGCAGCAGTA |
| Suggested primer 2: | CATGGTATCAGGCACACAGC |