| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.232955 |
| Chromosome: | chromosome 9 |
| Location: | 2788433 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389050 | CNX5,MAW4 | (1 of 2) PTHR16861//PTHR16861:SF4 - GLYCOPROTEIN 38 // PODOPLANIN; Membrane-associated hydroxyproline-rich glycoprotein 4 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGATGCTGTAACAGAGCATCCTTTTCGGA |
| Internal bar code: | GGTTAATCCCGGTCGCAGGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 405 |
| LEAP-Seq percent confirming: | 99.3678 |
| LEAP-Seq n confirming: | 2515 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTGATGTCGGGTGTTGTG |
| Suggested primer 2: | AGTTTGTCTGGTTCTGCGCT |