| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.232956 |
| Chromosome: | chromosome 8 |
| Location: | 5015114 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g386050 | (1 of 1) PTHR12917//PTHR12917:SF11 - ASPARTYL PROTEASE DDI-RELATED // RHOMBOID DOMAIN-CONTAINING PROTEIN 2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGGTGTGCGGGCACTATGCGCGGGCCCC |
| Internal bar code: | GGGGGAGTAAGCGGTCGTAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 578 |
| LEAP-Seq percent confirming: | 97.495 |
| LEAP-Seq n confirming: | 973 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTCCTACTTTGGTCCGTG |
| Suggested primer 2: | TAATGCGAGGCTCCTATGCT |