| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.232966 |
| Chromosome: | chromosome 6 |
| Location: | 7388274 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g299350 | FBB15 | Flagellar/basal body protein 15; (1 of 2) PF04819 - Family of unknown function (DUF716) (DUF716) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTTCCTGCTGTCATTCACTTCTATAGCC |
| Internal bar code: | GTGCAAGGGCTTTCTTTTCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 160 |
| LEAP-Seq percent confirming: | 99.3597 |
| LEAP-Seq n confirming: | 931 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCGTGTAGGGAAAGAAAG |
| Suggested primer 2: | CATGTTCGGAGATGACGATG |