| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.232976 |
| Chromosome: | chromosome 16 |
| Location: | 6109423 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g675851 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCTCAAATGCCGCAGCCCCACCTTTGGC |
| Internal bar code: | AATGTGGCGCATTCCGCGGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 430 |
| LEAP-Seq percent confirming: | 96.3006 |
| LEAP-Seq n confirming: | 19237 |
| LEAP-Seq n nonconfirming: | 739 |
| LEAP-Seq n unique pos: | 72 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTCGAATCGTATCACAGA |
| Suggested primer 2: | ATTGCTATTAGCCCCTCGGT |