| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.232983 |
| Chromosome: | chromosome 10 |
| Location: | 2731000 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g438350 | LTH3 | LAT2 homolog | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGCCCGGGATGCTCATGCACACGTGAGT |
| Internal bar code: | CATGATGTGTATTCGCAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 552 |
| LEAP-Seq percent confirming: | 84.6307 |
| LEAP-Seq n confirming: | 1696 |
| LEAP-Seq n nonconfirming: | 308 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGGACCGTTAGCTCTTCG |
| Suggested primer 2: | CTCACGCATGCTCCTAAACA |