| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.233005 |
| Chromosome: | chromosome 17 |
| Location: | 1770141 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g709300 | (1 of 1) PF10009 - Uncharacterized protein conserved in bacteria (DUF2252) (DUF2252) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGACGGGGGCTTGCTGCCGGGGTTGGT |
| Internal bar code: | GAGTAGATGGCGTAAGGGTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 673 |
| LEAP-Seq percent confirming: | 94.787 |
| LEAP-Seq n confirming: | 5764 |
| LEAP-Seq n nonconfirming: | 317 |
| LEAP-Seq n unique pos: | 66 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAACCCGTCTATCCTCGGT |
| Suggested primer 2: | ACCCACAACACTACGCACAA |