| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.233005 |
| Chromosome: | chromosome 17 |
| Location: | 2002813 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g711250 | MIP2 | (1 of 2) PTHR19139:SF180 - AQUAPORIN-1; Aquaporin | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCAAACTCCATAGAAACACCCACTACCC |
| Internal bar code: | GGCCTTGGGATAAAAATCGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 625 |
| LEAP-Seq percent confirming: | 97.9286 |
| LEAP-Seq n confirming: | 1371 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 99 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCTAGGGGGTCATACAGC |
| Suggested primer 2: | TTACAGGTGTGACCCATCCA |