Insertion junction: LMJ.RY0402.233017_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.233017
Chromosome:chromosome 17
Location:4644306
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g733250 FKB16D,FKB16-4,FKB7 (1 of 1) PTHR10516:SF323 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP16-4, CHLOROPLASTIC; peptidyl-prolyl cis-trans isomerase, FKBP-type 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCATCTGGCCACCCAGCAACCACACCAGC
Internal bar code:CGACGTACAGCCTGAACGGCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:772
LEAP-Seq percent confirming:98.9905
LEAP-Seq n confirming:6472
LEAP-Seq n nonconfirming:66
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:AAATGCTTTACCGTGAACCG
Suggested primer 2:GTACGGTAGCAACGGTTCGT