| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.233021 |
| Chromosome: | chromosome 9 |
| Location: | 877168 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401551 | BHD1 | 3-beta hydroxysteroid dehydrogenase/isomerase | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTTCTCCATGTAATTACCCCGGCCAAGA |
| Internal bar code: | CGGTCCGTCTTTTGACACCAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 448 |
| LEAP-Seq percent confirming: | 99.4624 |
| LEAP-Seq n confirming: | 7030 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACACGTTTGACTTCTGGGC |
| Suggested primer 2: | TGCAACACCAGTCAGCTTTC |