Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.233033 |
Chromosome: | chromosome_12 |
Location: | 7356095 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g557100 | antisense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AGACAGTGGTCCCGGAACGGTTTGCTAAAA |
Internal bar code: | TTGGAGCGCGTGTTGTGCAAAT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 773 |
LEAP-Seq percent confirming: | 98.7427 |
LEAP-Seq n confirming: | 1021 |
LEAP-Seq n nonconfirming: | 13 |
LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GATGACGAACTCACGCTTCA |
Suggested primer 2: | GGTGGTAAGCGGCACTATGT |