Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.233050 |
Chromosome: | chromosome_13 |
Location: | 1145680 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre13.g569550 | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTTGGTGAGGCTGGCAAAGTCGCTGGGGAT |
Internal bar code: | TGTAAGGTTGGTCGGCTGGATA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 649 |
LEAP-Seq percent confirming: | 98.7835 |
LEAP-Seq n confirming: | 10881 |
LEAP-Seq n nonconfirming: | 134 |
LEAP-Seq n unique pos: | 125 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTAGGCTGAAACACCACACG |
Suggested primer 2: | AAGGAGGTGGAGGTTGAGGT |