| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.233085 |
| Chromosome: | chromosome_2 |
| Location: | 2735719 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g093750 | NRX2 | Nucleoredoxin 2 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CATCTACGCCAACTTCAAGAAGGATCACGC |
| Internal bar code: | GAGGCTAAGCAGCGCGCCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1323 |
| LEAP-Seq percent confirming: | 99.0462 |
| LEAP-Seq n confirming: | 4777 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATAGCTTCTCCATAGCCG |
| Suggested primer 2: | CTAGAGCGATCGTCCCTTTG |