| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.233086 |
| Chromosome: | chromosome 7 |
| Location: | 5928062 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g354200 | GAP2 | Glyceraldehyde 3-phosphate dehydrogenase, GAPC, cytosolic; (1 of 2) 1.2.1.12 - Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) / Triosephosphate dehydrogenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAAAGGGAGTGCTATCGATGGTGCCACAC |
| Internal bar code: | GTAATGCACGTCGTCGTTTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 971 |
| LEAP-Seq percent confirming: | 99.4891 |
| LEAP-Seq n confirming: | 3700 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCAGCACCACATACACAAC |
| Suggested primer 2: | CGACAACGAGTACGGCTACA |