| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.233092 |
| Chromosome: | chromosome 6 |
| Location: | 5651696 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g286150 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGATATATACGAGCACTGGCTGCGCCCAC |
| Internal bar code: | CTCTTAATTTCGTGAGCGCATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 554 |
| LEAP-Seq percent confirming: | 99.7201 |
| LEAP-Seq n confirming: | 6056 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGGAGAGCAAACGGCAAG |
| Suggested primer 2: | GCACATTGTCCATTCGTCAC |