| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.233109 |
| Chromosome: | chromosome_16 |
| Location: | 6893922 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g676533 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGAGGTGTTGCTGCTCTTGCGGACGCCAGC |
| Internal bar code: | CGCGTAGGGGTGAGCTGTAAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 520 |
| LEAP-Seq percent confirming: | 85.5263 |
| LEAP-Seq n confirming: | 195 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAACAGGACAGCAGCACT |
| Suggested primer 2: | TCATCGTCGTCTGTCCTCTG |