| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.233151 |
| Chromosome: | chromosome 3 |
| Location: | 2940775 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g163200 | (1 of 1) K15683 - NF-X1-type zinc finger protein NFXL1 (NFXL1, OZFP) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCATGCAGCCCGGCCATCAGCTTTCGAT |
| Internal bar code: | ATGGCAGGTTTGCGGATTAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2146 |
| LEAP-Seq percent confirming: | 98.2059 |
| LEAP-Seq n confirming: | 2299 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATCAAGACTTCCTTGCCC |
| Suggested primer 2: | TAGGCTGATGGGCTAGCAGT |