| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.233161 |
| Chromosome: | chromosome 10 |
| Location: | 5691764 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g460600 | CYN53 | (1 of 2) PTHR11071//PTHR11071:SF297 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE // CYCLOPHILIN-LIKE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FAMILY PROTEIN; Cyclophilin 53 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGCATACACACACGCACAGGCCTCCCCG |
| Internal bar code: | GAGTGCCGCCAGCAGTATGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 988 |
| LEAP-Seq percent confirming: | 98.3227 |
| LEAP-Seq n confirming: | 6038 |
| LEAP-Seq n nonconfirming: | 103 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAAACACACAGAGGGAGGG |
| Suggested primer 2: | TGGTCACCCAACCAGTACCT |