| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.233168 |
| Chromosome: | chromosome 16 |
| Location: | 7286564 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g683595 | CAX4 | (1 of 1) PTHR12266//PTHR12266:SF0 - NA+/CA2+ K+ INDEPENDENT EXCHANGER // SODIUM/POTASSIUM/CALCIUM EXCHANGER 6, MITOCHONDRIAL; CAX family cation antiporter, membrane protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCATTCAATCCCACGCCACACCAACCCA |
| Internal bar code: | CATTGTATCATTGGCGTCTCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 22 |
| LEAP-Seq percent confirming: | 95.2381 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTATGATGGGGGTCGTGAT |
| Suggested primer 2: | GCCCTCACAGAAAGCTATGC |