Insertion junction: LMJ.RY0402.233191_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.233191
Chromosome:chromosome 4
Location:1226895
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g215250 FAP26 Ankyrin Repeat Flagellar Associated Protein 26; (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAGTACTTGGACGTTGTTGACAAGGCATA
Internal bar code:GTCGTGTGGTGTAGTGGAACCT

Confirmation - LEAP-Seq

LEAP-Seq distance:626
LEAP-Seq percent confirming:96.0849
LEAP-Seq n confirming:1448
LEAP-Seq n nonconfirming:59
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:GGAGAGGATTGGGAGAGGAC
Suggested primer 2:CATAGGTAGGAGCAGCGAGG