| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.233191 |
| Chromosome: | scaffold_18 |
| Location: | 95891 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre18.g748697 | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCCTGGCAGGCATGCCGGACTGGTGCAAGT |
| Internal bar code: | ATTGTAAGCGAGCGCGAATCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 629 |
| LEAP-Seq percent confirming: | 99.5088 |
| LEAP-Seq n confirming: | 6482 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCCATACAGCATATCACC |
| Suggested primer 2: | GCGTGTGTGTGTGTGTGTGT |