Insertion junction: LMJ.RY0402.233197_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.233197
Chromosome:chromosome 17
Location:2944359
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g719900 PWD1 Phosphoglucan water dikinase 1; (1 of 1) K15535 - phosphoglucan, water dikinase (PWD) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCCCGCGCACCGGTGCCACGTCACCCACT
Internal bar code:CGTAAGCGGGTCGCGTATGCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1111
LEAP-Seq percent confirming:99.4843
LEAP-Seq n confirming:6173
LEAP-Seq n nonconfirming:32
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CACCCCTGACCCTCTTTACA
Suggested primer 2:ATGACAATTTCCGAGGTTCG