| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.233259 |
| Chromosome: | chromosome 15 |
| Location: | 512743 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g636896 | (1 of 1) PTHR11082//PTHR11082:SF29 - TRNA-DIHYDROURIDINE SYNTHASE // TRNA-DIHYDROURIDINE SYNTHASE | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATCTCACTAACCTGGGGCAGCCGCAGTTC |
| Internal bar code: | TTAGCGTGTCGACAGTGACCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 384 |
| LEAP-Seq percent confirming: | 87.788 |
| LEAP-Seq n confirming: | 381 |
| LEAP-Seq n nonconfirming: | 53 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGAAGTGAAGGCGTAGGCA |
| Suggested primer 2: | GATGGCTAGCTAGGGCACTG |