| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.233273 |
| Chromosome: | chromosome 14 |
| Location: | 1459766 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g617950 | CAM18 | Calmodulin-like protein; (1 of 5) PTHR23050//PTHR23050:SF90 - CALCIUM BINDING PROTEIN // CENTRIN-3 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGCGGTCCATGGTGGCCTTGTTGAACAA |
| Internal bar code: | ATGTATAGGGATCTAGCGAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 181 |
| LEAP-Seq percent confirming: | 99.6974 |
| LEAP-Seq n confirming: | 1318 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGGTGCTAATTTGGGTGT |
| Suggested primer 2: | TCGCCCTCAAAAGCCTACTA |