| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.233303 |
| Chromosome: | chromosome 16 |
| Location: | 1677585 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g654600 | PHC56 | (1 of 71) PF12499 - Pherophorin (DUF3707); Pherophorin-chlamydomonas homolog 56 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCTGGCGGCCGTGCGCGCTGCACTCGCC |
| Internal bar code: | GGGTCAGAGGTGCTCAGGGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 643 |
| LEAP-Seq percent confirming: | 84.4444 |
| LEAP-Seq n confirming: | 38 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCCTGAAGGAAAAAGCAG |
| Suggested primer 2: | CGAACGTTGTCAAAACATGG |