| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.233351 |
| Chromosome: | chromosome 7 |
| Location: | 1436581 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g323100 | STK7,STPK7 | (1 of 1) K08853 - AP2-associated kinase [EC:2.7.11.1] (AAK); Serine/threonine protein kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGTGTGCTGGGGCTGAGGCCGCGGGCT |
| Internal bar code: | CCGGAACCACGCGCACTCCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 345 |
| LEAP-Seq percent confirming: | 98.8361 |
| LEAP-Seq n confirming: | 2123 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTATGGCTGCGGCTGTAGAT |
| Suggested primer 2: | GGGATTAGTGTCCACACGCT |