| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.233356 |
| Chromosome: | chromosome 13 |
| Location: | 3715154 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g589200 | (1 of 21) IPR011016 - Zinc finger, RING-CH-type | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTTACTGCTCGATAGCACGCCGCTGTCA |
| Internal bar code: | CGCTCGTGTGACATTTACTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 305 |
| LEAP-Seq percent confirming: | 99.6501 |
| LEAP-Seq n confirming: | 2563 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAGTAGCAGCACAGCAAG |
| Suggested primer 2: | ACAAACGGTTTCTTGTTGGC |