| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.233359 |
| Chromosome: | chromosome 12 |
| Location: | 6820075 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g561450 | (1 of 1) PTHR11228//PTHR11228:SF23 - RADICAL SAM DOMAIN PROTEIN // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAGTGGTGAACAACTTCAACGCCGGGGCG |
| Internal bar code: | CATATGCTCTGCCATCCGGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 919 |
| LEAP-Seq percent confirming: | 99.2551 |
| LEAP-Seq n confirming: | 533 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTGTCAACTGTGGGGATG |
| Suggested primer 2: | ATGAGGGTGTGTTGGGTGAT |