| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.233396 |
| Chromosome: | chromosome 2 |
| Location: | 2635054 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACACGCGACACAAAACACTGATAGCCAAG |
| Internal bar code: | AACTCAGTACCCGATTTTTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 734 |
| LEAP-Seq percent confirming: | 99.5955 |
| LEAP-Seq n confirming: | 4186 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGAGCAGTAGGGCTATCGT |
| Suggested primer 2: | TGTCATTCTGCCGGTTATGA |