| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.233435 |
| Chromosome: | chromosome 5 |
| Location: | 3336681 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g240650 | DNJ32 | (1 of 1) K09506 - DnaJ homolog subfamily A member 5 (DNAJA5); DnaJ-like protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGGGAGCCGGTCGCAGCTGTTCAAGCAT |
| Internal bar code: | CACATCTAGAGCACACTAAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 556 |
| LEAP-Seq percent confirming: | 99.2407 |
| LEAP-Seq n confirming: | 3006 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGATGAAGATGAGGATGCC |
| Suggested primer 2: | GGGAAAGAAGCACTTTGCTG |