| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.233440 |
| Chromosome: | chromosome 8 |
| Location: | 1949491 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g367500 | LHCSR3.1,LHCSR2 | Stress-related chlorophyll a/b binding protein 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCACGGAGCTGCCCCAGGGACTGACAAGA |
| Internal bar code: | GCGAATCATGCCGAAGTAGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 611 |
| LEAP-Seq percent confirming: | 98.9989 |
| LEAP-Seq n confirming: | 890 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGATGAGGGCAGAGGGTAA |
| Suggested primer 2: | TTCACACCCAATACCTGCAA |