Insertion junction: LMJ.RY0402.233457_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.233457
Chromosome:chromosome 4
Location:3924700
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g230928 TRL13 (1 of 1) K10758 - thiol oxidase (QSOX); Thioredoxin-like protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCTGTCGACCTGCTGCCGTACGGCAAGGC
Internal bar code:TCCGCGCTACCCATTTCGGCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:549
LEAP-Seq percent confirming:93.8804
LEAP-Seq n confirming:2025
LEAP-Seq n nonconfirming:132
LEAP-Seq n unique pos:51

Suggested primers for confirmation by PCR

Suggested primer 1:CAGGTACTGGTACACCGCCT
Suggested primer 2:CCATTGTAACATCGCCCTCT