Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.233460 |
Chromosome: | chromosome_12 |
Location: | 1900050 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g511050 | RSP16 | Radial spoke protein 16 | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCACATAGCAGCGGCTACCGTACTTTCATT |
Internal bar code: | AGATCCTGTGTCTCTGCGTGTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 928 |
LEAP-Seq percent confirming: | 94.9143 |
LEAP-Seq n confirming: | 3210 |
LEAP-Seq n nonconfirming: | 172 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATACCCACAGGGAAGGGAAC |
Suggested primer 2: | TACTCGAACAATGCAGGCAG |